WOW, this is a great depictable video about what goes on during expression and export of recombinant proteins. The things i really admired were:

1. How they captured and recognized the translated recombinant protein with it's N-Terminal Peptide Signal Sequence. Most videos neglect that.

2. How the Exportins recognized the N-Terminal Peptide Sequence (Nuclear export signals) of the recombinant protein, bound to it and aided in it's secretion from the nucleus through the receptors on the nuclear pore based on a particular pathway. Well Captured.

3. The cleavage of the N-Terminal Sequence after secretion of Rec.Protein from the nucleus.

4. How the Rec.Protein Maneuvers it's way through the reticulum and gets folded.
BUT they could have introduced chaperons to aid in the protein folding; proteins just don't fold on their own like that. Don't worry i know this is just a representation.

5. Recognition of the signal peptide containing the Twin-arginine Motif by the Taq Translocase recruited and brought together about seven protein receptors to aid in the secretion of a fully folded protein and after that the cleavage of the signal peptide. Well captured.

6. Excellent graphics,sound quality and an attractive voice narration. Beautiful.

Overall this video captured some hidden molecular steps that most videos neglect and videos like this can help those of us into molecular work visualize and pictures what we always assume and try to think is going on in a cell or a bacteria. For me in particular, these processes are all accurate and goes by the literature.

My Questions are:
1. Wouldn't the mass production of recombinant proteins from Corynex be in smaller quantities as compared to E.coli based on generation times? As much as i know, E.coli has a generation time of about 20 minutes and Corynebacterium glutamicum having between 90 minutes and 130 minutes based on a particular strain (a slow growth). This would make it produce Rec.proteins in low quantities as compared to E.coli unless there is a way to overcome that which i would really love to know.

2. E.coli is a natural inhabitant of the human gut but Corynebacterium glutamicum is not. What if Corynebacterium glutamicum finds it's way into the human gut after the administering of a Rec.protein intended for humans?? Anything can happen;they can be non-pathogenic but they don't exist there. Are there measures to check this???

Would really appreciate this Joel White if you can give me the answers to these questions.

My overall grade for this video is 90%. Excellent and i just love it. Keep up the good work.

Agyeman-Badu, Thankk you for taking the time to review the video and provide your feedback. The video file is rather large, so if anyone has trouble downloading and viewing, you can also access at www.corynex.com. You might also be interested in downloading a complementary whitepaper on “Speeding and Simplifying Recombinant Protein Production” – would appreciate any comments to this paper, as well.

I especially appreciate your thoughtful questions. Let me try to address at a high-level here, but I would be happy to discuss one-on-one as a follow-up. You can reach me directly at whitej@ajiusa.com or 919.723.2116.

At a very high level:

The generation time of the strains we developed is 30 to 35 minutes and can obtain high cell densities relatively quickly. In addition we use a very high expression promotor to further enhance expression levels.

We have been using the Cornynebacterium for almost 50 years to produce amino acids that are used as API's and in IV's. We also have Drug Master Files and safety data for items produced using the Corynex. We have validated methods for cell inactivation and removal.

Feel free to contact me directly to discuss further. Thanks again - Joel

Thank you, Erik for taking the time to review the video and provide your feedback. We had a great time putting this together - we worked with a great scientific animation company, XVIVO, you should check them out.

Don't mention it Joel, you welcome.
Was just amazed to realize that you can genetically manipulate an organism to have a shorter generation time and a very high promoter to enhance expression levels. You guys at Ajinomotor are really great. I can see a successful company with extremely smart scientists.